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Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
This content is Free Access.
1Centre de Recherche du Centre hospitalier de l’Université de Montréal (CRCHUM), 2Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine,Université de Montréal, 3Département de Pathologie et Biologie Cellulaire, Faculté de Médecine,Université de Montréal, 4Institut du Cancer de Montréal, 5Département de Neurosciences, Faculté de Médecine,Université de Montréal, 6Département de Chirurgie, Faculté de Médecine,Université de Montréal, 7Département de Médecine, Faculté de médecine,Université de Montréal
Chapters
- 00:05Introduction
- 01:03Heat-Induced Antigen Retrieval
- 01:46Nonspecific Binding Blocking
- 02:28Immunofluorescence Labeling
- 03:36Image Alignment
- 04:36Tissue Detection
- 05:35Tissue Segmentation
- 06:28Cell of Interest (COI) Identification and Quantification
- 07:15Tissue Heatmapping
- 08:10Results: Representative Visualization, Quantification, and Mapping of Cell Populations of Interest in the Tumor Microenvironment
- 10:02Conclusion
Here, we describe a simple and accessible strategy for visualizing, quantifying, and mapping immune cells in formalin-fixed paraffin-embedded tumor tissue sections. This methodology combines existing imaging and digital analysis techniques with the purpose of expanding the multiplexing capability and multiparameter analysis of imaging assays.
