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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
 

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Article DOI: 10.3791/60740-v • 11:00 min • March 25th, 2020
March 25th, 2020 •
1,2, 1,2, 1, 1,3, 1,4, 1,2, 1,5, 1,4,6, 1,4,7
1Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), 2Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, 3Département de Pathologie et Biologie Cellulaire, Faculté de Médecine, Université de Montréal, 4Institut du Cancer de Montréal, 5Département de Neurosciences, Faculté de Médecine, Université de Montréal, 6Département de Chirurgie, Faculté de Médecine, Université de Montréal, 7Département de Médecine, Faculté de médecine, Université de Montréal

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résumé
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Here, we describe a simple and accessible strategy for visualizing, quantifying, and mapping immune cells in formalin-fixed paraffin-embedded tumor tissue sections. This methodology combines existing imaging and digital analysis techniques with the purpose of expanding the multiplexing capability and multiparameter analysis of imaging assays.

Tags

Visualization Quantification Mapping Immune Cell Populations Tumor Microenvironment Clinical Value Immunotherapy Cancer Treatment Tissue-immune Signatures Antibody Combinations Stripping Techniques APPs (antibody Panel Protocols) Image Analysis Methods Technical Language Video Demonstration Antigen Retrieval Formalin-fixed Paraffin-embedded Tumor Tissue Sections Coplin Jar Antigen Retrieval Solution Electric Pressure Cooker
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