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February 18, 2020
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1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Harvard University, 2Department of Biology,College of William and Mary, 3Harvard Medical School,Harvard University, 4Department of Bioengineering,California Institute of Technology, 5Department of Computer Science,College of William and Mary
Summary
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蛍光カルシウムイメージングとin situハイブリダイゼーションを組み合わせた2部構成のプロトコルを提示し、実験者がカルシウム活性のパターンを単一細胞レベルの遺伝子発現プロファイルと相関させることが可能です。
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Cite this Article
Ablondi, E. F., Paudel, S., Sehdev, M., Marken, J. P., Halleran, A. D., Rahman, A., Kemper, P., Saha, M. S. Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis. J. Vis. Exp. (156), e60726, doi:10.3791/60726 (2020).
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