Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Ravian L. van Ineveld; Hendrikus C.R. Ariese; Ellen J. Wehrens; Johanna F. Dekkers; Anne C. Rios

doi:10.3791/60709

Journal

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Biology

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Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

JoVE Journal
Biology

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JoVE Journal Biology

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

14,982 Views

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10:40 min

•

June 05, 2020

DOI:

10.3791/60709-v

DOI:

10.3791/60709-v

•

10:40 min

June 05, 2020

•

44 Views

Ravian L. van Ineveld*^1,2,3, Hendrikus C.R. Ariese*^1,2,3, Ellen J. Wehrens^1,2,3, Johanna F. Dekkers*^1,2,3,4, Anne C. Rios*^1,2,3

¹Princess Máxima Center for Pediatric Oncology, ²Department of Cancer Research, Oncode Institute,Hubrecht Institute-KNAW Utrecht, ³Cancer Genomics Center (CGC), ⁴Hubrecht Institute,Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht

Summary

The entire 3D structure and cellular content of organoids, as well as their phenotypic resemblance to the original tissue can be captured using the single-cell resolution 3D imaging protocol described here. This protocol can be applied to a wide range of organoids varying in origin, size and shape.

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Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

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