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Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Journal JoVE
Biologie

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Journal JoVE Biologie

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

14,706 Views

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10:40 min

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June 05, 2020

DOI:

10.3791/60709-v

DOI:

10.3791/60709-v

•

10:40 min

June 05, 2020

•

10 Views

Ravian L. van Ineveld*^1,2,3, Hendrikus C.R. Ariese*^1,2,3, Ellen J. Wehrens^2,3, Johanna F. Dekkers*^1,2,3,4, Anne C. Rios*^1,2,3

¹Princess Máxima Center for Pediatric Oncology, ²Department of Cancer Research, Oncode Institute,Hubrecht Institute-KNAW Utrecht, ³Cancer Genomics Center (CGC), ⁴Hubrecht Institute,Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht

Tags

Organoids 3D Imaging Single-cell Resolution Optical Clearing Sample Preparation Basement Membrane Extract Recovery Solution PBS-BSA Centrifugation Paraformaldehyde PBS-Tween 20 Organoid Washing Buffer

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