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Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
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Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

14,981 Views

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10:40 min

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June 05, 2020

DOI:

10.3791/60709-v

DOI:
10.3791/60709-v

•

10:40 min
June 05, 2020

•

44 Views
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1Princess Máxima Center for Pediatric Oncology, 2Department of Cancer Research, Oncode Institute,Hubrecht Institute-KNAW Utrecht, 3Cancer Genomics Center (CGC), 4Hubrecht Institute,Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht

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The entire 3D structure and cellular content of organoids, as well as their phenotypic resemblance to the original tissue can be captured using the single-cell resolution 3D imaging protocol described here. This protocol can be applied to a wide range of organoids varying in origin, size and shape.

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van Ineveld, R. L., Ariese, H. C., Wehrens, E. J., Dekkers, J. F., Rios, A. C. Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids. J. Vis. Exp. (160), e60709, doi:10.3791/60709 (2020).

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