Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Ravian L. van Ineveld; Hendrikus C.R. Ariese; Ellen J. Wehrens; Johanna F. Dekkers; Anne C. Rios

doi:10.3791/60709

Journal

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生物学

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Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

14,981 Views

•

10:40 min

•

June 05, 2020

DOI:

10.3791/60709-v

DOI:

10.3791/60709-v

•

10:40 min

June 05, 2020

•

44 Views

Ravian L. van Ineveld*^1,2,3, Hendrikus C.R. Ariese*^1,2,3, Ellen J. Wehrens^1,2,3, Johanna F. Dekkers*^1,2,3,4, Anne C. Rios*^1,2,3

¹Princess Máxima Center for Pediatric Oncology, ²Department of Cancer Research, Oncode Institute,Hubrecht Institute-KNAW Utrecht, ³Cancer Genomics Center (CGC), ⁴Hubrecht Institute,Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht

Summary

The entire 3D structure and cellular content of organoids, as well as their phenotypic resemblance to the original tissue can be captured using the single-cell resolution 3D imaging protocol described here. This protocol can be applied to a wide range of organoids varying in origin, size and shape.

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Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Summary

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