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Volledig verwerkte recombinant KRAS4b: isoleren en karakteriseren van het Farnesylated en gemethyleerd eiwit
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Biochemistry
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JoVE Journal Biochemistry
Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein
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Volledig verwerkte recombinant KRAS4b: isoleren en karakteriseren van het Farnesylated en gemethyleerd eiwit

Nederlands

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5,496 Views

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07:08 min

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January 16, 2020

DOI:

10.3791/60703-v

DOI:
10.3791/60703-v

•

07:08 min
January 16, 2020

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10 Views
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1NCI RAS Initiative, Cancer Research Technology Program,Frederick National Laboratory for Cancer Research

Summary

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Prenylatie is een belangrijke modificatie op perifere membraan bindende eiwitten. Insecten cellen kunnen worden gemanipuleerd om gefarnesylen carboxymethylated KRAS4b te produceren in hoeveelheden die biofysische metingen van eiwit-eiwit-en proteïne-lipide-interacties mogelijk maken

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Cite this Article

Agamasu, C., Frank, P., Perkins, S., Waybright, T., Messing, S., Gillette, W., Stephen, A. G. Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein. J. Vis. Exp. (155), e60703, doi:10.3791/60703 (2020).

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