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Journal
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Biochemistry
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完全加工重组KRAS4b：分离和描述脱脂和甲基化蛋白
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein
Please note that all translations are automatically generated.
Click here for the English version.
完全加工重组KRAS4b：分离和描述脱脂和甲基化蛋白
DOI:
10.3791/60703-v
•
07:08 min
•
January 16, 2020
•
Constance Agamasu
,
Peter Frank
,
Shelley Perkins
,
Timothy Waybright
,
Simon Messing
,
William Gillette
,
Andrew G. Stephen
1
NCI RAS Initiative, Cancer Research Technology Program
,
Frederick National Laboratory for Cancer Research
Chapters
00:05
Title
01:30
Protein Purification
04:20
Results: Purification and Analysis of KRAS4b Proteins
06:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
前膜化是外周膜结合蛋白的重要修饰。昆虫细胞可以纵产生脱脂和甲苯甲酸化 KRAS4b 的数量，使生物物理测量蛋白质 - 蛋白质和蛋白质 - 脂质相互作用
Tags
KRAS4b
Farnesylation
Methylation
Recombinant Protein
Protein Purification
Cation Exchange Chromatography
Baculovirus Expression
Cancer
Biophysical Experiments
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