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Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein
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Biochemistry
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JoVE Journal Biochemistry
Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein

Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein

English

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5,496 Views

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07:08 min

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January 16, 2020

DOI:

10.3791/60703-v

DOI:
10.3791/60703-v

•

07:08 min
January 16, 2020

•

10 Views
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1NCI RAS Initiative, Cancer Research Technology Program,Frederick National Laboratory for Cancer Research

Summary

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Prenylation is an important modification on peripheral membrane binding proteins. Insect cells can be manipulated to produce farnesylated and carboxymethylated KRAS4b in quantities that enable biophysical measurements of protein-protein and protein-lipid interactions

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Cite this Article

Agamasu, C., Frank, P., Perkins, S., Waybright, T., Messing, S., Gillette, W., Stephen, A. G. Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein. J. Vis. Exp. (155), e60703, doi:10.3791/60703 (2020).

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