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Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein
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Biochimica
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JoVE Journal Biochimica
Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein

Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein

DOI:
10.3791/60703-v

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07:08 min

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January 16, 2020

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, , , , , ,
1NCI RAS Initiative, Cancer Research Technology Program,Frederick National Laboratory for Cancer Research

Capitoli

  • 00:05Titolo
  • 01:30Protein Purification
  • 04:20Results: Purification and Analysis of KRAS4b Proteins
  • 06:14Conclusion

Summary

Traduzione automatica

Traduzione automatica

Prenylation is an important modification on peripheral membrane binding proteins. Insect cells can be manipulated to produce farnesylated and carboxymethylated KRAS4b in quantities that enable biophysical measurements of protein-protein and protein-lipid interactions

Tags

KRAS4bFarnesylationMethylationRecombinant ProteinProtein PurificationCation Exchange ChromatographyBaculovirus ExpressionCancerBiophysical Experiments

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