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Journal
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Biochemistry
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PeptiQuick,将膜蛋白一步进生物仿入肽,用于简化蛋白质结合测定
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays
Please note that all translations are automatically generated.
Click here for the English version.
PeptiQuick,将膜蛋白一步进生物仿入肽,用于简化蛋白质结合测定
DOI:
10.3791/60661-v
•
15:04 min
•
November 02, 2019
•
James W. Saville
,
Luca A. Troman
,
Franck Duong Van Hoa
1
Department of Biochemistry
,
University of British Columbia
,
2
School of Biochemistry
,
University of Bristol
Chapters
00:01
Title
03:48
Protocol
12:19
Results
14:19
Conclusion
Summary
Automatic Translation
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我们提出了一种方法,结合膜蛋白纯化和重组到消化片在一个单一的色谱步骤。生物素氨酸脚手架用于通过生物层干涉测量直接表面附着和蛋白质-配体相互作用的测量。
Tags
Membrane Proteins
Peptidisc
Biotinylated Peptidiscs
Protein Binding Assays
Detergent-free
Bio-layer Interferometry
FhuA
Outer Membrane Vesicles
PeptiQuick Reconstitution
Nickel-NTA IMAC
Biotinylated Peptide
Streptavidin Coated Sensors
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