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PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays
JoVE Journal
Biochimica
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JoVE Journal Biochimica
PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

10,443 Views

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15:04 min

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November 02, 2019

DOI:

10.3791/60661-v

DOI:
10.3791/60661-v

•

15:04 min
November 02, 2019

•

5 Views
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1Department of Biochemistry,University of British Columbia, 2School of Biochemistry,University of Bristol

Summary

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We present a method that combines membrane protein purification and reconstitution into peptidiscs in a single chromatographic step. Biotinylated scaffolds are used for direct surface attachment and measurement of protein-ligand interactions via biolayer interferometry.

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Cite this Article

Saville, J. W., Troman, L. A., Duong Van Hoa, F. PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays. J. Vis. Exp. (153), e60661, doi:10.3791/60661 (2019).

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