Journal
/
Biochimie
/
PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays
Journal JoVE
Biochimie
Author Produced
This content is Free Access.
Journal JoVE Biochimie
PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

10,443 Views

•

15:04 min

•

November 02, 2019

DOI:

10.3791/60661-v

DOI:
10.3791/60661-v

•

15:04 min
November 02, 2019

•

5 Views
, ,
1Department of Biochemistry,University of British Columbia, 2School of Biochemistry,University of Bristol

Summary

Automatically generated

We present a method that combines membrane protein purification and reconstitution into peptidiscs in a single chromatographic step. Biotinylated scaffolds are used for direct surface attachment and measurement of protein-ligand interactions via biolayer interferometry.

Explore More Videos

Membrane ProteinsPeptidiscBiotinylated PeptidiscsProtein Binding AssaysDetergent-freeBio-layer InterferometryFhuAOuter Membrane VesiclesPeptiQuick ReconstitutionNickel-NTA IMACBiotinylated PeptideStreptavidin Coated Sensors

Read Article

Cite this Article

Saville, J. W., Troman, L. A., Duong Van Hoa, F. PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays. J. Vis. Exp. (153), e60661, doi:10.3791/60661 (2019).

Download .ris file

Copy

Share Video

.

Copy