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Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
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Biochemistry
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JoVE Journal Biochemistry
Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

DOI:
10.3791/59538-v

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11:59 min

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May 15, 2019

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, ,
1Department of Microbiology and Molecular Genetics,Michigan State University, 2Department of Physiology,Michigan State University

Chapters

  • 00:04Title
  • 01:01Fractionation of LDL (Low-density Lipoprotein) Containing Plasma from Chicken Egg Yolk
  • 02:04Isolation of LDL Particles from Plasma
  • 03:16Incubation of S. aureus with LDLs for Membrane Lipid Analysis
  • 05:18Extraction of S. aureus Membrane Lipids
  • 06:54Analysis of S. aureus Lipid Profiles using High Resolution/Accurate Mass Spectrometry
  • 08:26Database Searching to Identify Endogenous S. aureus and Exogenous LDL-derived Lipids
  • 09:54Results: Fatty Acid Composition of S. aureus Phospholipids Cultured with Chicken LDLs
  • 11:29Conclusion

Summary

Automatic Translation

Automatic Translation

This method provides a framework for studying incorporation of exogenous fatty acids from complex host sources into bacterial membranes, particularly Staphylococcus aureus. To achieve this, protocols for the enrichment of lipoprotein particles from chicken egg yolk and subsequent fatty acid profiling of bacterial phospholipids utilizing mass spectrometry are described.

Tags

Lipoprotein ParticlesChicken Egg YolkBacterial PathogenFatty Acid IncorporationMembrane PhospholipidsLipidomic AnalysisStaphylococcus AureusAmmonium SulfateDialysis

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