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Diferenciação neural eficiente usando a cultura unicelular de células-tronco embrionárias humanas
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Diferenciação neural eficiente usando a cultura unicelular de células-tronco embrionárias humanas

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9,669 Views

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11:17 min

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January 18, 2020

DOI:

10.3791/60571-v

DOI:
10.3791/60571-v

•

11:17 min
January 18, 2020

•

2 Views
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1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences,National Institutes of Health, 2NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke,National Institutes of Health

Summary

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Apresentado aqui é um protocolo para a geração de uma cultura unicelular de células-tronco embrionárias humanas e sua diferenciação subseqüente em células progenitoras neurais. O protocolo é simples, robusto, escalável e adequado para rastreamento de medicamentos e aplicações de medicina regenerativa.

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Cite this Article

Jeon, K., Park, K., Jetten, A. M. Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells. J. Vis. Exp. (155), e60571, doi:10.3791/60571 (2020).

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