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ヒト胚性幹細胞の単細胞培養を用いた効率的な神経分化
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
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ヒト胚性幹細胞の単細胞培養を用いた効率的な神経分化

日本語

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9,669 Views

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11:17 min

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January 18, 2020

DOI:

10.3791/60571-v

DOI:
10.3791/60571-v

•

11:17 min
January 18, 2020

•

2 Views
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1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences,National Institutes of Health, 2NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke,National Institutes of Health

Summary

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ここで提示するプロトコルは、ヒト胚性幹細胞の単細胞培養の生成とその後の神経前駆細胞への分化のためのプロトコルである。プロトコルは、シンプルで堅牢でスケーラブルで、薬物スクリーニングや再生医療用途に適しています。

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Cite this Article

Jeon, K., Park, K., Jetten, A. M. Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells. J. Vis. Exp. (155), e60571, doi:10.3791/60571 (2020).

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