Journal
/
Biología del desarrollo
/
Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

9,666 Views

•

11:17 min

•

January 18, 2020

DOI:

10.3791/60571-v

DOI:
10.3791/60571-v

•

11:17 min
January 18, 2020

•

2 Views
, ,
1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences,National Institutes of Health, 2NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke,National Institutes of Health

Summary

Automatically generated

Presented here is a protocol for the generation of a single-cell culture of human embryonic stem cells and their subsequent differentiation into neural progenitor cells. The protocol is simple, robust, scalable, and suitable for drug screening and regenerative medicine applications.

Explore More Videos

Human Embryonic Stem CellsNeural Progenitor CellsSingle-cell CultureEfficient DifferentiationScalable Culture SystemMulti-step DifferentiationNeural LineagesBasement Membrane MatrixCell DetachmentMTeSR-1 Medium

Read Article

Cite this Article

Jeon, K., Park, K., Jetten, A. M. Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells. J. Vis. Exp. (155), e60571, doi:10.3791/60571 (2020).

Download .ris file

Copy

Share Video

.

Copy