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Effektiv neural differensiering bruker single-Cell kultur av Human embryonale stamceller
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
DOI:

11:17 min

January 18, 2020

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Chapters

  • 00:05Title
  • 01:05Adaptation of Colony Type hESCs to Single-cell hESC Culture
  • 04:01Embryoid Body Formation and Differentiation
  • 05:44NPC Expansion and Cryopreservation
  • 06:41Differentiation of NPCs into Dopaminergic Neurons and Astrocytes
  • 08:54Results: In vitro Differentiation of Adapted Single-cell type hESCs
  • 10:34Conclusion

Summary

Automatic Translation

Presentert her er en protokoll for generering av en enkelt cellekultur av menneskelig embryonale stamceller og deres påfølgende differensiering inn nevrale stamceller. Protokollen er enkel, robust, skalerbar, og egnet for narkotika screening og regenererende medisin applikasjoner.

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