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Prenatal Islmn'den Okülomotor, Troklear ve Spinal Motor Nöronların İzolasyon ve Kültürü :GFP Transgenik Fareler
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November 12, 2019
DOI:
1Department of Neurology,Boston Children’s Hospital, 2FM Kirby Neurobiology Center,Boston Children’s Hospital, 3Department of Neurology,Harvard Medical School, 4Medical Genetics Training Program,Harvard Medical School, 5Department of Ophthalmology,Boston Children’s Hospital, 6Department of Ophthalmology,Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology,Kokura Memorial Hospital, 10Department of Genetics,Albert Einstein College of Medicine
Summary
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Bu çalışma, primer okülomotor, troklear ve spinal motor nöronların homojen hücre kültürlerini ortaya çıkarmak için bir protokol sunmaktadır. Bu kültürler, oküler ve spinal motor nöronların morfolojik, hücresel, moleküler ve elektrofizyolojik özelliklerinin karşılaştırmalı analizleri için kullanılabilir.
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Cite this Article
Fujiki, R., Lee, J. Y., Jurgens, J. A., Whitman, M. C., Engle, E. C. Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice. J. Vis. Exp. (153), e60440, doi:10.3791/60440 (2019).
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