/
/
Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice
10,566 Views
•
10:04 min
•
November 12, 2019
DOI:
1Department of Neurology,Boston Children’s Hospital, 2FM Kirby Neurobiology Center,Boston Children’s Hospital, 3Department of Neurology,Harvard Medical School, 4Medical Genetics Training Program,Harvard Medical School, 5Department of Ophthalmology,Boston Children’s Hospital, 6Department of Ophthalmology,Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology,Kokura Memorial Hospital, 10Department of Genetics,Albert Einstein College of Medicine
Summary
Automatically generated
This work presents a protocol to yield homogeneous cell cultures of primary oculomotor, trochlear, and spinal motor neurons. These cultures can be used for comparative analyses of the morphological, cellular, molecular, and electrophysiological characteristics of ocular and spinal motor neurons.
Read Article
Cite this Article
Fujiki, R., Lee, J. Y., Jurgens, J. A., Whitman, M. C., Engle, E. C. Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice. J. Vis. Exp. (153), e60440, doi:10.3791/60440 (2019).
Copy