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Isolamento e Cultura dos neurônios oculomotores, trochlear e motores espinhais de pré-natal Islmn:GFP Ratos Transgênicos
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10:04 min
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November 12, 2019
DOI:
1Department of Neurology,Boston Children’s Hospital, 2FM Kirby Neurobiology Center,Boston Children’s Hospital, 3Department of Neurology,Harvard Medical School, 4Medical Genetics Training Program,Harvard Medical School, 5Department of Ophthalmology,Boston Children’s Hospital, 6Department of Ophthalmology,Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology,Kokura Memorial Hospital, 10Department of Genetics,Albert Einstein College of Medicine
Summary
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Este trabalho apresenta um protocolo para produzir culturas celulares homogêneas de neurônios motores oculomotores primários, trochlear e espinhais. Essas culturas podem ser usadas para análises comparativas das características morfológicas, celulares, moleculares e eletrofisiológicas dos neurônios motores oculares e espinhais.
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Fujiki, R., Lee, J. Y., Jurgens, J. A., Whitman, M. C., Engle, E. C. Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice. J. Vis. Exp. (153), e60440, doi:10.3791/60440 (2019).
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