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Charakterisierung von funktional assoziierten miRNAs in Glioblastom und deren Engineering in künstliche Cluster für die Gentherapie
JoVE Journal
Genetics
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JoVE Journal Genetics
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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Charakterisierung von funktional assoziierten miRNAs in Glioblastom und deren Engineering in künstliche Cluster für die Gentherapie

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5,507 Views

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09:40 min

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October 04, 2019

DOI:

10.3791/60215-v

DOI:
10.3791/60215-v

•

09:40 min
October 04, 2019

•

1 Views
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1Department of Neurosurgery,Brigham and Women’s Hospital-Harvard Medical School

Summary

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Hier wird ein Protokoll zur Charakterisierung von Modulen biologisch synergistischer miRNAs und deren Montage in kurze Transgene beschrieben, das eine gleichzeitige Überexpression für Gentherapieanwendungen ermöglicht.

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Cite this Article

Bhaskaran, V., Peruzzi, P. Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy. J. Vis. Exp. (152), e60215, doi:10.3791/60215 (2019).

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