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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
JoVE Journal
Genetica
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JoVE Journal Genetica
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

5,507 Views

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09:40 min

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October 04, 2019

DOI:

10.3791/60215-v

DOI:
10.3791/60215-v

•

09:40 min
October 04, 2019

•

1 Views
,
1Department of Neurosurgery,Brigham and Women’s Hospital-Harvard Medical School

Summary

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Described here is a protocol for characterizing modules of biologically synergistic miRNAs and their assembly into short transgenes, which allows simultaneous overexpression for gene therapy applications.

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MiRNAMicroRNAGlioblastomaGene TherapyArtificial MiRNA ClustersNeural Stem CellsGBM-34 Glioblastoma Stem-like CellsTemozolomideBioinformaticsGene ExpressionCancerPoly-D-lysineCell CultureIn Vitro

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Cite this Article

Bhaskaran, V., Peruzzi, P. Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy. J. Vis. Exp. (152), e60215, doi:10.3791/60215 (2019).

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