Described here is a protocol for characterizing modules of biologically synergistic miRNAs and their assembly into short transgenes, which allows simultaneous overexpression for gene therapy applications.
Bhaskaran, V., Peruzzi, P. Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy. J. Vis. Exp. (152), e60215, doi:10.3791/60215 (2019).