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08:09 min
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October 26, 2019
DOI:
1Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences,University of Manitoba, 2The Children’s Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences,University of Manitoba, 3Department of Oral Biology,University of Illinois at Chicago, 4Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences,University of Manitoba
Summary
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Realizar experimentos in vitro para reflejar las condiciones in vivo lo más adecuadamente posible no es una tarea fácil. El uso de cultivos celulares primarios es un paso importante hacia la comprensión de la biología celular en todo un organismo. El protocolo proporcionado describe cómo crecer y cultivar con éxito las neuronas cerebelosas de ratón embrionarios.
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Cite this Article
Shabanipour, S., Dalvand, A., Jiao, X., Rahimi Balaei, M., Chung, S. H., Kong, J., Del Bigio, M. R., Marzban, H. Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum. J. Vis. Exp. (152), e60168, doi:10.3791/60168 (2019).
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