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October 26, 2019
DOI:
1Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences,University of Manitoba, 2The Children’s Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences,University of Manitoba, 3Department of Oral Biology,University of Illinois at Chicago, 4Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences,University of Manitoba
Summary
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Condurre esperimenti in vitro per riflettere le condizioni in vivo nel modo più adeguato possibile non è un compito facile. L'uso delle colture cellulari primarie è un passo importante verso la comprensione della biologia cellulare in un intero organismo. Il protocollo fornito descrive come crescere e coltivare neuroni cerebellari embrionali del topo.
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Shabanipour, S., Dalvand, A., Jiao, X., Rahimi Balaei, M., Chung, S. H., Kong, J., Del Bigio, M. R., Marzban, H. Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum. J. Vis. Exp. (152), e60168, doi:10.3791/60168 (2019).
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