JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum
18,647 Views
•
08:09 min
•
October 26, 2019
DOI:
1Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences,University of Manitoba, 2The Children’s Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences,University of Manitoba, 3Department of Oral Biology,University of Illinois at Chicago, 4Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences,University of Manitoba
Summary
Automatically generated
Conducting in vitro experiments to reflect in vivo conditions as adequately as possible is not an easy task. The use of primary cell cultures is an important step toward understanding cell biology in a whole organism. The provided protocol outlines how to successfully grow and culture embryonic mouse cerebellar neurons.
Read Article
Cite this Article
Shabanipour, S., Dalvand, A., Jiao, X., Rahimi Balaei, M., Chung, S. H., Kong, J., Del Bigio, M. R., Marzban, H. Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum. J. Vis. Exp. (152), e60168, doi:10.3791/60168 (2019).
Copy