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CRISPR-Cas9を用いたミツバチ脳部における抗RDLおよび抗mGlutR1受容体抗体検査
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9
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CRISPR-Cas9を用いたミツバチ脳部における抗RDLおよび抗mGlutR1受容体抗体検査

日本語

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7,818 Views

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09:25 min

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January 30, 2020

DOI:

10.3791/59993-v

DOI:
10.3791/59993-v

•

09:25 min
January 30, 2020

•

4 Views
, , , , , , , ,
1Department of Neuroscience,University of Arizona, 2School of Life Sciences,Arizona State University, 3Department of Scientific and Technologic Investigations,University of Sonora, 4Integrated DNA Technologies, Inc.

Summary

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ここでは、成人ミツバチの脳におけるタンパク質の産生を減らして抗体特異性をテストするためにCRISPR-Cas9システムを使用するプロトコルです。

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Cite this Article

Sinakevitch, I., Kurtzman, Z., Choi, H. G., Ruiz Pardo, D. A., Dahan, R. A., Klein, N., Bugarija, B., Wendlandt, E., Smith, B. H. Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9. J. Vis. Exp. (155), e59993, doi:10.3791/59993 (2020).

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