Presented here is a protocol to use the CRISPR-Cas9 system for reducing the production of a protein in the adult honeybee brain to test antibody specificity.
Sinakevitch, I., Kurtzman, Z., Choi, H. G., Ruiz Pardo, D. A., Dahan, R. A., Klein, N., Bugarija, B., Wendlandt, E., Smith, B. H. Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9. J. Vis. Exp. (155), e59993, doi:10.3791/59993 (2020).