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Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9
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Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9

Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9

7,818 Views

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09:25 min

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January 30, 2020

DOI:

10.3791/59993-v

DOI:
10.3791/59993-v

•

09:25 min
January 30, 2020

•

4 Views
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1Nörobilim Bölümü,University of Arizona, 2School of Life Sciences,Arizona State University, 3Department of Scientific and Technologic Investigations,University of Sonora, 4Integrated DNA Technologies, Inc.

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Presented here is a protocol to use the CRISPR-Cas9 system for reducing the production of a protein in the adult honeybee brain to test antibody specificity.

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Cite this Article

Sinakevitch, I., Kurtzman, Z., Choi, H. G., Ruiz Pardo, D. A., Dahan, R. A., Klein, N., Bugarija, B., Wendlandt, E., Smith, B. H. Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9. J. Vis. Exp. (155), e59993, doi:10.3791/59993 (2020).

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