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Neuroscience

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JoVE Journal Neuroscience
Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9
 

Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9

Article DOI: 10.3791/59993-v • 09:25 min • January 30th, 2020
January 30th, 2020 •
1,2, *2, *2, 3, 2, 1, 4, 4, 2
1Department of Neuroscience, University of Arizona, 2School of Life Sciences, Arizona State University, 3Department of Scientific and Technologic Investigations, University of Sonora, 4Integrated DNA Technologies, Inc.
* These authors contributed equally

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Resumo
Automatic Translation

Presented here is a protocol to use the CRISPR-Cas9 system for reducing the production of a protein in the adult honeybee brain to test antibody specificity.

Tags

Anti-RDL Anti-mGlutR1 Antibody Testing Honeybee Brain Sections CRISPR-Cas9 Gene Editing Ionotropic GABAA Receptors Metabotropic Glutamate Receptors Learning And Memory Laboratory Conditions Specificity Of Antibodies Immunocytochemical Analysis Guide RNA Complex Formations Nucleotide-free Buffer
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