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January 21, 2020
DOI:
1Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences, 2State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agricultural Sciences and School of Life Sciences,Peking University
Summary
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A bioinformatics pipeline, namely miRDeep-P2 (miRDP2 for short), with updated plant miRNA criteria and an overhauled algorithm, could accurately and efficiently analyze microRNA transcriptomes in plants, especially for species with complex and large genomes.
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Bioinformatics PipelineMicroRNA TranscriptomesPlantsMiRDeep2Plant MicroRNAsTranscriptional RegulatorsPlant DevelopmentEnvironmental ChallengesFalse PositivesProcessing TimeHigh Confidence MicroRNA AnnotationGenome FunctionMiRDeep2 PipelineArabidopsis ThalianaGenome Reference IndexNoncoding RNA Reference IndexDeep Sequencing ReadsAdaptersFASTA Identifiers
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Cite this Article
Wang, Y., Kuang, Z., Li, L., Yang, X. A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants. J. Vis. Exp. (155), e59864, doi:10.3791/59864 (2020).
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