Journal
/
遗传学
/
A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
JoVE 杂志
遗传学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 遗传学
A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

DOI:
10.3791/59864-v

•

06:34 min

•

January 21, 2020

•

, , ,
1Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences, 2State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agricultural Sciences and School of Life Sciences,Peking University

Chapters

  • 00:04Title
  • 01:15Installation and Testing
  • 02:56Identifying Novel MicroRNAs (miRNAs)
  • 03:33Reference Index Building
  • 04:42Results: Representative miRDP2 Performance
  • 05:45Conclusion

Summary

自动翻译

自动翻译

A bioinformatics pipeline, namely miRDeep-P2 (miRDP2 for short), with updated plant miRNA criteria and an overhauled algorithm, could accurately and efficiently analyze microRNA transcriptomes in plants, especially for species with complex and large genomes.

Tags

Bioinformatics PipelineMicroRNA TranscriptomesPlantsMiRDeep2Plant MicroRNAsTranscriptional RegulatorsPlant DevelopmentEnvironmental ChallengesFalse PositivesProcessing TimeHigh Confidence MicroRNA AnnotationGenome FunctionMiRDeep2 PipelineArabidopsis ThalianaGenome Reference IndexNoncoding RNA Reference IndexDeep Sequencing ReadsAdaptersFASTA Identifiers

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Detection of Copy Number Alterations Using Single Cell Sequencing

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Read Article