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Immunology and Infection

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毛里小肠内皮淋巴细胞的内质成像

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Immunology and Infection

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JoVE Journal Immunology and Infection

Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

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毛里小肠内皮淋巴细胞的内质成像

DOI:

10.3791/59853-v

•

08:00 min

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June 24, 2019

•

Luo Jia, Karen L. Edelblum

¹Center for Immunity and Inflammation, Department of Pathology, Immunology and Laboratory Medicine,Rutgers New Jersey Medical School

Chapters

00:04Title
00:58Intestinal Mucosa Exposure
03:41Spinning Disk Confocal Microscopy Imaging
05:59Results: Representative Gamma-delta Intraepithelial Lymphocyte (IEL) Behavior Analyses
07:15Conclusion

Summary

Automatic Translation

我们描述了一种通过倒转旋转盘共聚焦显微镜,利用鼠小肠的细胞内成像来可视化GFP标记的IELs的方法。该技术能够跟踪粘液中的活细胞长达4小时,并可用于研究各种肠道免疫-皮皮相互作用。

Tags

Intravital Imaging Intraepithelial Lymphocytes Murine Small Intestine Microscopy Immune Cell Interactions Epithelium Real-time Imaging Mucosal Immunity Epithelial Biology Cancer Biology Surgical Technique Anesthetized Transgenic Mouse EGFP Gamma-delta T-cell Receptor Hoechst 33342 Dye Abdominal Incision Mesentery Peritoneal Cavity Suture Placement

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