Journal
/
/
使用新激酶蛋白的强生化方法在分子水平上进行表征
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
DOI:

11:23 min

June 30, 2019

, , ,

Chapters

  • 00:04Title
  • 01:24Transient Transfections
  • 03:21Lysis
  • 05:17Immunoprecipitation
  • 06:59Coimmunoprecipitation Analyses
  • 08:33Results: Synthesized LIMK2-1 Protein, Its Interaction with Kinase ROCK, its Kinase Activity
  • 10:10Conclusion

Summary

Automatic Translation

我们使用强大的生化方法对一种新的激酶蛋白进行了定性:用特殊抗体对不同细胞系和组织进行西带体分析,通过共免疫沉淀实验相互作用,由西方检测到的激酶活性使用磷特异性抗体和由 β+32P+ ATP 标记的斑点。

Related Videos

Read Article