需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
6,046 Views
•
11:23 min
•
June 30, 2019
DOI:
Summary
Automatically generated
We characterized a new kinase protein using robust biochemical approaches: Western Blot analysis with a dedicated specific antibody on different cell lines and tissues, interactions by coimmunoprecipitation experiments, kinase activity detected by Western Blot using a phospho-specific antibody and by γ[32P] ATP labeling.
Read Article
Cite this Article
Vallée, B., Doudeau, M., Godin, F., Bénédetti, H. Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein. J. Vis. Exp. (148), e59820, doi:10.3791/59820 (2019).
Copy