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Immunology and Infection
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明確に定義されたせん断応力下の単一細胞イオン電流の電気生理学的記録
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JoVE Journal
Immunology and Infection
Electrophysiological Recordings of Single-cell Ion Currents Under Well-defined Shear Stress
Please note that all translations are automatically generated.
Click here for the English version.
明確に定義されたせん断応力下の単一細胞イオン電流の電気生理学的記録
DOI:
10.3791/59776-v
•
07:17 min
•
August 02, 2019
•
Ibra S. Fancher
,
Irena Levitan
1
Department of Medicine, Division of Pulmonary, Critical Care, Sleep and Allergy
,
University of Illinois at Chicago
Chapters
00:04
Title
00:29
Assembly of the Modified Parallel Plate Flow Chamber
01:43
Cell Preparation and Seeding into the MPP Flow Chamber
03:43
Controlling Shear Stress to the MPP Flow Chamber for Electrophysiological Recordings
05:46
Results: Example of Leak Subtraction for Accurate Analysis of Mechano-activated Kir Current
06:43
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルの目的は、せん断応力によるメカノ感受性イオンチャネルのリアルタイム活性化を調査する上で使用する改変並列プレートフローチャンバを記述することです。
Tags
Electrophysiological Recordings
Single-cell Ion Currents
Shear Stress
Parallel Plate Flow Chamber
Mechanosensitive Ion Channels
Silicone Elastomer
Cover Glass
Cell Culture
Electrophysiological BAF Solution
Cell Adhesion
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