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Electrophysiological Recordings of Single-cell Ion Currents Under Well-defined Shear Stress
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JoVE 杂志 免疫与感染
Electrophysiological Recordings of Single-cell Ion Currents Under Well-defined Shear Stress

Electrophysiological Recordings of Single-cell Ion Currents Under Well-defined Shear Stress

DOI:
10.3791/59776-v

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07:17 min

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August 02, 2019

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,
1Department of Medicine, Division of Pulmonary, Critical Care, Sleep and Allergy,University of Illinois at Chicago

Chapters

  • 00:04Title
  • 00:29Assembly of the Modified Parallel Plate Flow Chamber
  • 01:43Cell Preparation and Seeding into the MPP Flow Chamber
  • 03:43Controlling Shear Stress to the MPP Flow Chamber for Electrophysiological Recordings
  • 05:46Results: Example of Leak Subtraction for Accurate Analysis of Mechano-activated Kir Current
  • 06:43Conclusion

Summary

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The goal of this protocol is to describe a modified parallel plate flow chamber for use in investigating real time activation of mechanosensitive ion channels by shear stress.

Tags

Electrophysiological RecordingsSingle-cell Ion CurrentsShear StressParallel Plate Flow ChamberMechanosensitive Ion ChannelsSilicone ElastomerCover GlassCell CultureElectrophysiological BAF SolutionCell Adhesion

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