Journal
/
Immunology and Infection
/
Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex

Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex

English

Automatically Generated

11,542 Views

•

09:23 min

•

June 30, 2019

DOI:

10.3791/59703-v

DOI:
10.3791/59703-v

•

09:23 min
June 30, 2019

•

12 Views
, , , , , ,
1Section of Pediatric Infectious Diseases and Allergy, Department of Medicine,Imperial College London, 2Department of Pharmacy,National University of Singapore, 3MRC Centre for Molecular Bacteriology and Infection, Department of Medicine,Imperial College London

Summary

Automatically generated

Galleria mellonella was recently established as a reproducible, cheap, and ethically acceptable infection model for the Mycobacterium tuberculosis complex. Here we describe and demonstrate the steps taken to establish successful infection of G. mellonella with bioluminescent Mycobacterium bovis BCG lux.

Explore More Videos

Galleria MellonellaTuberculosisInfection ModelMycobacteriumInnate ImmunityAlternative To Mammalian ModelsBioluminescenceBCGCell CultureSample Preparation

Read Article

Cite this Article

Asai, M., Li, Y., Khara, J. S., Gladstone, C. A., Robertson, B. D., Langford, P. R., Newton, S. M. Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex. J. Vis. Exp. (148), e59703, doi:10.3791/59703 (2019).

Download .ris file

Copy

Share Video

.

Copy