Journal
/
Immunologie und Infektion
/
Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex

Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex

DOI:
10.3791/59703-v

•

09:23 min

•

June 30, 2019

•

, , , , , ,
1Section of Pediatric Infectious Diseases and Allergy, Department of Medicine,Imperial College London, 2Department of Pharmacy,National University of Singapore, 3MRC Centre for Molecular Bacteriology and Infection, Department of Medicine,Imperial College London

Kapitel

  • 00:04Titel
  • 00:59Preparation of M. bovis BCG lux for Infection
  • 03:09Infecting G. mellonella with BCG lux
  • 05:23Measuring the In Vivo Burden of BCG lux in G. mellonella
  • 07:10Results: BCG lux Dose Dependent Virulence
  • 08:31Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Galleria mellonella was recently established as a reproducible, cheap, and ethically acceptable infection model for the Mycobacterium tuberculosis complex. Here we describe and demonstrate the steps taken to establish successful infection of G. mellonella with bioluminescent Mycobacterium bovis BCG lux.

Tags

Galleria MellonellaTuberculosisInfection ModelMycobacteriumInnate ImmunityAlternative To Mammalian ModelsBioluminescenceBCGCell CultureSample Preparation

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Introducing Shear Stress in the Study of Bacterial Adhesion

Growth of Mycobacterium tuberculosis Biofilms

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis

An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei

Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery

A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Read Article