需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex
Chapters
- 00:04Title
- 00:59Preparation of M. bovis BCG lux for Infection
- 03:09Infecting G. mellonella with BCG lux
- 05:23Measuring the In Vivo Burden of BCG lux in G. mellonella
- 07:10Results: BCG lux Dose Dependent Virulence
- 08:31Conclusion
Galleria mellonella was recently established as a reproducible, cheap, and ethically acceptable infection model for the Mycobacterium tuberculosis complex. Here we describe and demonstrate the steps taken to establish successful infection of G. mellonella with bioluminescent Mycobacterium bovis BCG lux.
