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KSHV感染细胞中特定基因产物的定量原位荧光杂交（FISH）和免疫荧光（IF）
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells
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KSHV感染细胞中特定基因产物的定量原位荧光杂交（FISH）和免疫荧光（IF）

DOI:
10.3791/59697-v

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00:06 min

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August 27, 2019

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,
1Norfolk Academy, 2Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute,Yale School of Medicine

Chapters

  • 00:04Title
  • 00:28Cell Fixation, Immunofluorescence, and Hybridization, and Viral RNA Visualization
  • 04:48Results: Representative FISH and IF Kaposi s Sarcoma-Associated Herpesvirus (KSHV) Oligonucleotide Analyses
  • 05:52Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种利用荧光原位杂交（FISH）来可视化被神经感染的人体细胞中的多种疱疹病毒RNA的协议，无论是悬浮的还是粘附的。该协议包括荧光的定量，产生核细胞质比，并可扩展用于同时可视化具有免疫荧光（IF）的宿主和病毒蛋白。

Tags

Quantitative FISHImmunofluorescenceKSHVHost-virus InteractionsViral Gene ProductsRNAProteinBiochemical AssaysCell CultureFixationPermeabilizationBlockingPrimary AntibodySecondary AntibodyFluorescent LabelingHybridizationOligonucleotidesDenaturation

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