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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
 

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

Article DOI: 10.3791/59626-v • 08:58 min • May 1st, 2019
May 1st, 2019 •
1, 1, 1, 1
1Division of Biology, Kansas State University

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Automatic Translation

We present a protocol to study mRNA translation regulation in poxvirus-infected cells using in vitro Transcribed RNA-based luciferase reporter assay. The assay can be used for studying translation regulation by cis-elements of an mRNA, including 5’-untranslated region (UTR) and 3’-UTR. Different translation initiation modes can also be examined using this method.

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Keywords: In Vitro Transcribed RNA Luciferase Reporter Assay Translation Regulation Poxvirus-infected Cells Poly(A) Tail 5'UTR 3'UTR Cap Modification PCR Amplicon T7 Promoter Firefly Luciferase Renilla Luciferase Kozak Sequence Agarose Gel Electrophoresis
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