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Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

8,602 Views

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07:20 min

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April 09, 2019

DOI:

10.3791/59357-v

DOI:
10.3791/59357-v

•

07:20 min
April 09, 2019

•

1 Views
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1Department of Internal Medicine,University of New Mexico Health Science Center, 2Department of Medicine, Division of Gastroenterology & Hepatology,Johns Hopkins University School of Medicine

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Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.

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Colonoid MonolayersIntestinal EpitheliumHost-pathogen InteractionMucus BiologyMicrobiomeInflammatory Bowel Disease3D Organoid CultureEx Vivo StudiesHarvesting SolutionCell SuspensionDMEM/F12 MediumCell PlatingExpansion Medium

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In, J. G., Foulke-Abel, J., Clarke, E., Kovbasnjuk, O. Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium. J. Vis. Exp. (146), e59357, doi:10.3791/59357 (2019).

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