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Immunology and Infection
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Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Studying Organelle Dynamics in B Cells During Immune Synapse Formation

Studying Organelle Dynamics in B Cells During Immune Synapse Formation

English

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8,776 Views

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15:39 min

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June 01, 2019

DOI:

10.3791/59621-v

DOI:
10.3791/59621-v

•

15:39 min
June 01, 2019

•

8 Views
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1Laboratory of Immune Cell Biology, Department of Cellular and Molecular Biology,Pontificia Universidad Católica de Chile, 2Faculty of Medicine,Pontificia Universidad Católica de Chile, 3Centro de Investigaciones en Biología Celular y Biomedicina, Facultad de Ciencia,Universidad San Sebastián

Summary

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Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.

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Ibañez-Vega, J., Fuentes, D., Lagos, J., Cancino, J., Yuseff, M. I. Studying Organelle Dynamics in B Cells During Immune Synapse Formation. J. Vis. Exp. (148), e59621, doi:10.3791/59621 (2019).

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