/
/
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
8,776 Views
•
15:39 min
•
June 01, 2019
DOI:
Summary
Automatically generated
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
Read Article
Cite this Article
Ibañez-Vega, J., Fuentes, D., Lagos, J., Cancino, J., Yuseff, M. I. Studying Organelle Dynamics in B Cells During Immune Synapse Formation. J. Vis. Exp. (148), e59621, doi:10.3791/59621 (2019).
Copy