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Målretning Alpha Synuclein aggregater i kutane perifer nerve fibre af Free-flydende immunofluorescens analyse
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JoVE Journal Neuroscience
Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay

Målretning Alpha Synuclein aggregater i kutane perifer nerve fibre af Free-flydende immunofluorescens analyse

DOI:
10.3791/59558-v

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08:33 min

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June 25, 2019

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, , ,
1Laboratory for Biomedical Neurosciences,Neurocenter of Southern Switzerland, 2Neurology Department,Neurocenter of Southern Switzerland, 3Department of Neurology, Inselspital, Bern University Hospital,University of Bern, 4Faculty of Biomedical Sciences,Università della Svizzera Italiana

Chapters

  • 00:04Title
  • 01:17Tissue Fixation and Storage
  • 02:15Tissue Cut with a Cryotome
  • 03:11Immunofluorescence Staining
  • 05:34Immunofluorescence Imaging
  • 06:01Results: Analysis of the Free-floating Immunofluorescence Assay
  • 08:09Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi en protokol for en fritflydende indirekte immunofluorescens assay på hud biopsi sektioner, der giver mulighed for identifikation af sygdomsspecifikke kropsbygnings varianter af alpha synuclein involveret i Parkinsons sygdom og flere proteiner i perifert nervesystem.

Tags

Parkinson’s DiseaseSkin BiopsyAlpha Synuclein AggregatesPeripheral Nerve FibersImmunofluorescence AssayCryoprotectantCryosectioningBiomarkerPathogenesis

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