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Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils
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JoVE Journal Neuroscience
Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils

Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils

DOI:
10.3791/55503-v

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09:32 min

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April 13, 2017

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, , , , ,
1German Center for Neurodegenerative Diseases (DZNE), 2Centre for Prions and Protein Folding Diseases & Department of Biochemistry,University of Alberta

Chapters

  • 00:05Title
  • 00:57Intraglossal Injection of Alpha-synuclein Fibrils
  • 02:26Intraperitoneal Injections
  • 03:18Bioluminescence Imaging
  • 06:16Results: Injection wth Alph-synuclein Fibrils via the Intraperitoneal or Intraglossal Route Causes Disease in Biogenic Tg(M83+/-:Gfap-luc+/- Mice
  • 08:28Conclusion

Summary

Automatic Translation

Automatic Translation

Peripheral injection of alpha-synuclein fibrils into the peritoneum or tongue of Tg(M83+/-:Gfap-luc+/-) mice, which express human alpha-synuclein with the familial A53T mutation and firefly luciferase, can induce neuropathology, including neuroinflammation, in their central nervous system.

Tags

Bioluminescence ImagingNeuroinflammationTransgenic MiceAlpha-synuclein FibrilsParkinson’s DiseaseNoninvasive MonitoringAnesthesiaIntranasal InoculationIntraperitoneal InjectionNerve InflammationNeurological Disease

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