Journal
/
Immunology and Infection
/
Usando organóides intestinais derivados de células-tronco pluripotentes induzidas por humanos para estudar e modificar a proteção de células epiteliais contra Salmonella e outros patógenos
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

Usando organóides intestinais derivados de células-tronco pluripotentes induzidas por humanos para estudar e modificar a proteção de células epiteliais contra Salmonella e outros patógenos

português

Automatically Generated

9,298 Views

•

10:59 min

•

May 12, 2019

DOI:

10.3791/59478-v

DOI:
10.3791/59478-v

•

10:59 min
May 12, 2019

•

3 Views
, , , , ,
1Wellcome Trust Sanger Institute, 2Department of Medicine,University of Cambridge, 3University of Cardiff

Explore More Videos

Human Induced Pluripotent Stem CellsIntestinal OrganoidsEpithelial Cell ProtectionSalmonellaPathogensMicro-injectionDifferentiationRPMI-B27 MediaCHIR99021Retinoic AcidBasement Membrane MatrixCollagenaseOrganoid Base Growth MediaCentrifugation24-well Plate

Read Article

Cite this Article

Lees, E. A., Forbester, J. L., Forrest, S., Kane, L., Goulding, D., Dougan, G. Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens. J. Vis. Exp. (147), e59478, doi:10.3791/59478 (2019).

Download .ris file

Copy

Share Video

.

Copy